Naked-eye sensitive detection of nuclease activity using positively-charged gold nanoparticles as colorimetric probes.
نویسندگان
چکیده
Positively-charged gold nanoparticles can effectively differentiate long DNA and fragmented DNA, thus providing a simple and visual approach to colorimetric detection of nuclease activity.
منابع مشابه
Sensitive and selective detection of cysteine using gold nanoparticles as colorimetric probes.
We report herein the development of a highly sensitive and selective colorimetric detection method for cysteine using gold nanoparticles probes. This assay relies upon the distance-dependent optical properties of gold nanoparticles, the self-assembly of cysteine on gold nanoparticles, and the interaction of a 2:1 cysteine/Cu2+ complex. In the presence of Cu2+, cysteine could rapidly induce the ...
متن کاملGold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor.
By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor (FR). A detection limit of 50 fM FR was obtained using UV-vis spectrometry and 10 pM FR could be visualized by the naked eye.
متن کاملLabel-free and naked eye detection of PNA/DNA hybridization using enhancement of gold nanoparticles.
Utilizing a gold enhancement process after inducing electrostatic interaction between positively charged gold nanoparticles and negatively charged target DNA hybridized to neutral PNA capture probes, a new method for label-free detection of DNA was developed and successfully applied to detect H5-type DNA.
متن کاملSensitive detection of microRNAs based on the conversion of colorimetric assay into electrochemical analysis with duplex-specific nuclease-assisted signal amplification
miRNAs have emerged as new biomarkers for the detection of a wide variety of cancers. By employing duplex-specific nuclease for signal amplification and gold nanoparticles (AuNPs) as the carriers of detection probes, a novel electrochemical assay of miRNAs was performed. The method is based on conversion of the well-known colorimetric assay into electrochemical analysis with enhanced sensitivit...
متن کاملHighly Sensitive Colorimetric Biosensor for Staphylococcal Enterotoxin B by a Label-Free Aptamer and Gold Nanoparticles
A simple, sensitive and selective colorimetric biosensor for the detection of Staphylococcal enterotoxin B (SEB) was developed using SEB-binding aptamer (SEB2) as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric probes. The assay is based on color change from red to purple due to conformational change of aptamer in the presence of SEB, and the phenomenon of salt-ind...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Chemical communications
دوره 47 45 شماره
صفحات -
تاریخ انتشار 2011